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Science of FluoDiving

Night dives with a UV/blue torch for viewing bio-fluorescence, the property of some marine life to re-emit light with a longer wavelength (of visible light) when illuminated with (nearly invisible) UV/blue light. In the following sections we will explain the science behind underwater fluorescence in general as well as the science behind our products in particular.

 

What is the allure?

The sight is magical, enchanting - as if the underwater life was actively shining like neon signs in the dark, or like a psychedelic disco. It is discovering a hidden world behind a hidden world.

A great deal of research has gone into the development of these products and we are certain you will enjoy the results of your fluo diving experience. Fire Dive Gear prides itself on offering some of the finest quality dive gear and photography products at the most reasonable prices. All our fluo diving hardware is fine-tuned against calibrated instrumentation, machined at close tolerance and uses the highest quality LED's and filter materials available. We offer custom mask filters and made-to-order camera and strobe filters.

 

Fluorescent Anemone in Roatan, Honduras

The photo shows a fluorescent anemone. Copyright 2014 Lynn Miner : Roatan Honduras

What is fluorescence diving?

The purpose of this discussion is to present the science (at a very cursory level) behind how our products work in terms of causing corals and other sea creatures to fluoresce. A few technical terms have been included in this text so the more advanced reader can refer to their search engine of choice and delve deeper into the technical aspects of this science. However, we have tried to keep the hypertechnoverbalizationalisms ;-) to a minimum for the lay reader.

The Physics of Light

We must start our discussion with a look at the electromagnetic spectrum (EM), see the figure below.

Fg14_17The visible portion of the EM spectrum is a very narrow band of wavelengths from about 400 to 700 nanometers (nm or 1x10-9meters). The area we are most concerned with is what is called near ultra violet (UV) or "actinic" light. This is light whose peak intensity is centered around a wavelength at approximately 420 nm.  All radiative energy has an associated wavelength (λ) and and frequency (f).

One is a function of the other: f=C/λ and λ=C/f, where C is the speed of light (3x108 meters per second, or m/s).

Viewing the figure above, wavelength is the distance in meters from one peak (or any other reference point on a wave) to the next peak (or next reference point) along the x-axis.  Frequency is simply the number of times that wavelength occurs in one second and has an inverse relationship to wavelength.  The higher the frequency, the shorter the wavelength and the higher the associated energy.  Inversely, the longer the wavelength, the lower the frequency with associated lower energy (all other factors being equal).  The y-axis is called the amplitude of the waveform.

The term "actinic" is somewhat of a throwback to the early days of photography. Early films were not sensitive to red light and hence the term "safe lights" refers to dim red lights which were used in darkrooms during processing. Films of that era were sensitive to blue or "actinic" light, however. The term "actinic" is also used in many scientific papers on the subject of coral fluorescence. Referring again to the figure above, you see that this is in the deep blue, nearly violet portion of the spectrum.

As all divers know, blue is the last color of the spectrum to be absorbed by water, hence near-actinic light is the light most sea creatures are exposed to, and that is also the light which is available for their photosynthesis and that which they have evolved under over the eons of time..

Referring again to the figure of the electromagnetic spectrum above, note that as one moves to the right of the scale, the energy levels increase. Therefore UV light has more energy than red light. This will play an important role in our discussion below.

Please also refer to the above figure whenever you see references to wavelength and frequency throughout this discussion.

A "Full Spectrum Light" is a light source which emits all the wavelengths of the visible spectrum in the same proportions as natural sunlight.

A lamp, LED, or bulb labeled "full spectrum" means that it emits light over the entire visible spectrum with a spectral output similar to that of the sun. This is what a standard white light underwater dive light (or torch) produces.

What we as divers need to have in order for the effect of fluorescence to occur and in order to be able to observe it are two things:

  1. A light source that emits light near or slightly above the actinic band (~430-450 nm). This can be a blue LED, or a dichroic filter which blocks everything from a white light source except blue, or preferably a combination of both a blue LED and the filter. This "assembly" we shall refer to as the excitation source or exciter. This exciter is used to shine on the subject target organisms.
  2. A second filter is needed to block the blue light from coming back into your eyes. This is called a barrier filter and is made of a yellow acrylic material which blocks the intense blue light reflected from the target, and which at the same time passes the emission light (fluorescence) in the green to red range. This filter is placed over your dive mask (and/or camera lens).

RadiationIntensityVSWavelength

Above is a graph representing the output of the sun (solar irradiance).  Note where the peak amplitude is - right there at the actinic band of wavelengths.  As a side note, the numbers at the top of the figure represent the percentages of energy emitted by the sun at various wavelengths.  About 7% is in the UV range, 44% is visible light and the remaining 49% is infrared and beyond.  That last 49% is felt by you as warmth.

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